This is a contentious issue, relating to knowledge of animal behavior, evolution, genetics, and various other aspects.
Animal Behavior and Evolution
To answer this question, we first need to understand the behavior and evolution of animals. Animal behavior is the result of their adaptation and survival, influenced by both genetic and environmental factors. Evolution is the process by which organisms evolve to adapt to the environment, primarily through the mechanism of natural selection.
Wolves are wild animals with strong consciousness of family and social behavior. They live in the wild and need to cooperate with each other for hunting, reproduction, and territory protection.
In wolf packs, there is a strict hierarchical system, with female wolves often becoming leaders, leading the pack and maintaining territory and pack order.

Dogs, close relatives of wolves, have been domesticated by humans for a long time and have become cherished companions. Compared to wolves, dogs have lost many of their wild traits and original social behaviors, but still retain some social skills and group recognition.
From the above characteristics, it can be seen that wolves and dogs are both highly social animals, with behaviors and evolutionary processes sharing many similarities but also exhibiting some differences.
Experimentation and Behavioral Studies
To explore whether wolves consider puppy dogs as their own kin, scientists have conducted a series of experiments and studies on behavior. Below, we will explore the results and findings of these experiments and studies.

Experiment 1: A Study by Canadian Researchers
In one experiment, Canadian researchers placed a puppy dog next to a newly born female wolf in a brand-new wolf den. The researchers observed the interaction and behavior between the wolf and the puppy dog.
Observations indicate that female wolves do not treat puppy dogs as their own offspring. Conversely, female wolves display agitation and refusal to a certain extent; they will attempt to use force to drive the puppy dogs out of the wolf den. In some cases, female wolves may even bite the puppy dogs, exhibiting aggressive behavior.
The conclusion of this experiment is that wolves do not regard puppy dogs as their own kin.

Experiment 2: Study by Italian Researchers
Italian researchers utilized a medium-sized dog and an Arctic wolf in their experiments. They placed newly born puppy dogs between the wolf and the dog, then observed their interaction and behavior.
Results showed that the Arctic wolf displayed some curious behaviors but did not exhibit any maternal behaviors. Instead, they showed aggressive behavior, even biting the puppy dogs in some instances.
However, when researchers separated the puppy dogs from the medium-sized dogs in two separate enclosures, the Arctic wolves displayed certain reactions, such as sniffing the puppy dogs and circling around the front of the enclosure. This indicates that Arctic wolves may perceive the presence of other animals around them but do not regard the puppy dogs as their own offspring.
From the results of these experiments and studies, it can be seen that wolves do not consider puppy dogs as their own offspring. Their behavior and expression towards puppy dogs differ from those towards their own offspring.

Why do wolf dogs not regard puppy dogs as their biological offspring?
This relates to why animals evolve and behave the way they do. The behavior and evolution of wolves are influenced by various factors such as environment, resources, genetics... They are a relatively primitive species with strong wild instincts and family bonds.
In the wild, wolves need to cooperate with each other for hunting, breeding, and protecting their territory.

Dogs are close relatives of wolves, but when humans domesticate and breed them, their genes and behaviors gradually change. Humans have selected and bred dogs for social behavior, cognition, and emotional expression different from those of wolves in the wild. Thus, the behavior and evolution of dogs and wolves have been differently impacted.
As for wolves, they exhibit great fierceness when their offspring and territory are threatened. When wolves encounter creatures like puppy dogs, they do not perceive them as their own offspring but rather as rival competitors or potential prey.
Furthermore, dogs and wolves differ in their social hierarchy system and behavior. In nature, wolves establish social structures and rules through kinship relations, status, and behavior among groups. Dogs have the ability to form close relationships with humans and play various roles in families and societies.
Therefore, the behavior and evolution between wolves and dogs exhibit significant differences. Wolves will not regard puppy dogs as their biological offspring, due to differences in behavioral habits and ecological adaptation mechanisms among different species in the wild.
