To diagnose urinary infections or kidney diseases in children, obtaining a urine sample is crucial. Since infections are common in children, ensuring a clean and accurate sample collection is essential. While older children can use the 'clean-catch' method, infants require the 'bag specimen' method. Collecting uncontaminated samples from young girls demands extra care due to anatomical challenges. Contamination leads to false-positive results, potentially leading to unnecessary treatments or invasive tests.
Steps
Using the Clean-Catch Method


- If feasible, bathe her earlier in the day and clean her genitalia with soap and water.
- Encourage her to urinate at the first sensation, not when urgency sets in.

- Lather thoroughly, including between fingers and underneath fingernails, for at least 20 seconds.
- Consider using alcohol-based sanitizer for added cleanliness.
- Avoid touching your mouth or face after sanitizing your hands and before cleaning your daughter.

- Use separate wipes for different areas to prevent cross-contamination.
- Avoid wiping from bottom to top to prevent bacterial transfer from the anus.

- Avoid touching the cup's interior when opening it.
- Place the cup upside down on a clean surface until ready for use.
- If a sterilized cup is unavailable, boil a small glass jar and lid for about 10 minutes, then air dry before use.

- If necessary, stimulate urination by running water.
- Collect midstream urine for optimal sample quality.

- If at the doctor's office, hand the sample to the nurse or assistant.
- If at home, refrigerate the sample and deliver it within 24 hours to prevent bacterial growth.
Bag Specimen Method

- The bag fits under the diaper and collects urine.
- Though prone to contamination, bag samples offer insight into your child's urinary health.

- Bathe her and clean her genitalia before attempting to collect urine.
- Offer liquids to stimulate urination after bathing.

- Do not apply sanitizer to your infant's genitalia; use antibacterial wipes instead.
- Apply a second round of sanitizer for added safety.

- You may wear gloves, but it's optional.
- Wipe only in one direction, avoiding circular motions.
- Avoid wiping from the anus to the vagina to prevent bacterial transfer.

- Always cover the bag with a clean diaper to prevent leaks.
- Check your infant regularly to monitor urine output.
- Be prepared for the bag to shift due to your infant's movement.

- Prior to removing the bag, prepare the cup by placing its lid upside down on a paper towel.
- Avoid touching the inside of the cup or lid during transfer.
Tips
- If using the bag method, there's minimal risk to your infant, though adhesive irritation is possible. Consider alternative methods if needed.
- Consult your doctor about using a toilet hat for urine collection if necessary for accuracy.
- Store urine samples in the fridge if not immediately sent to the lab, ensuring use within 24 hours to prevent contamination.
Essential Supplies
- Sanitized collection cup
- Antibacterial wet wipes
- Bottle of hand sanitizer
- Paper towels
- Vinyl or latex medical gloves
- Urine collection bag